Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin.
Identifieur interne : 002F01 ( Main/Exploration ); précédent : 002F00; suivant : 002F02Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin.
Auteurs : Seiji Nakagame [Canada] ; Richard P. Chandra ; John F. Kadla ; Jack N. SaddlerSource :
- Biotechnology and bioengineering [ 1097-0290 ] ; 2011.
Descripteurs français
- KwdFr :
- Acides carboxyliques (analyse), Biomasse (MeSH), Biotechnologie (méthodes), Cellulases (métabolisme), Hydrolyse (MeSH), Lignine (composition chimique), Lignine (isolement et purification), Lignine (métabolisme), Pinus (composition chimique), Pinus (métabolisme), Populus (composition chimique), Populus (métabolisme), Spectroscopie infrarouge à transformée de Fourier (MeSH), Spectroscopie par résonance magnétique (MeSH), Zea mays (composition chimique), Zea mays (métabolisme).
- MESH :
- analyse : Acides carboxyliques.
- composition chimique : Lignine, Pinus, Populus, Zea mays.
- isolement et purification : Lignine.
- métabolisme : Cellulases, Lignine, Pinus, Populus, Zea mays.
- méthodes : Biotechnologie.
- Biomasse, Hydrolyse, Spectroscopie infrarouge à transformée de Fourier, Spectroscopie par résonance magnétique.
English descriptors
- KwdEn :
- Biomass (MeSH), Biotechnology (methods), Carboxylic Acids (analysis), Cellulases (metabolism), Hydrolysis (MeSH), Lignin (chemistry), Lignin (isolation & purification), Lignin (metabolism), Magnetic Resonance Spectroscopy (MeSH), Pinus (chemistry), Pinus (metabolism), Populus (chemistry), Populus (metabolism), Spectroscopy, Fourier Transform Infrared (MeSH), Zea mays (chemistry), Zea mays (metabolism).
- MESH :
- chemical , analysis : Carboxylic Acids.
- chemical , chemistry : Lignin.
- chemical , isolation & purification : Lignin.
- chemical , metabolism : Cellulases, Lignin.
- chemistry : Pinus, Populus, Zea mays.
- metabolism : Pinus, Populus, Zea mays.
- methods : Biotechnology.
- Biomass, Hydrolysis, Magnetic Resonance Spectroscopy, Spectroscopy, Fourier Transform Infrared.
Abstract
To assess the effects that the physical and chemical properties of lignin might have on the enzymatic hydrolysis of pretreated lignocellulosic substrates, protease treated lignin (PTL) and cellulolytic enzyme lignin (CEL) fractions, isolated from steam and organosolv pretreated corn stover, poplar, and lodgepole pine, were prepared and characterized. The adsorption of cellulases to the isolated lignin preparations corresponded to a Langmuir adsorption isotherm. It was apparent that, rather than the physical properties of the isolated lignin, the carboxylic acid functionality of the isolated lignin, as determined by FTIR and NMR spectroscopy, had much more of an influence when lignin was added to typical hydrolysis of pure cellulose (Avicel). An increase in the carboxylic content of the lignin preparation resulted in an increased hydrolysis yield. These results suggested that the carboxylic acids within the lignin partially alleviate non-productive binding of cellulases to lignin. To try to confirm this possible mechanism, dehydrogenative polymers (DHP) of monolignols were synthesized from coniferyl alcohol (CA) and ferulic acid (FA), and these model compounds were added to a typical enzymatic hydrolysis of Avicel. The DHP from FA, which was enriched in carboxylic acid groups compared with the DHP from CA, adsorbed a lower mount of cellulases and did not decrease hydrolysis yields when compared to the DHP from CA, which decreased the hydrolysis of Avicel by 8.4%. Thus, increasing the carboxylic acid content of the lignin seemed to significantly decrease the non-productive binding of cellulases and consequently increased the enzymatic hydrolysis of the cellulose.
DOI: 10.1002/bit.22981
PubMed: 21246506
Affiliations:
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Le document en format XML
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Chandra</name></author><author><name sortKey="Kadla, John F" sort="Kadla, John F" uniqKey="Kadla J" first="John F" last="Kadla">John F. Kadla</name></author><author><name sortKey="Saddler, Jack N" sort="Saddler, Jack N" uniqKey="Saddler J" first="Jack N" last="Saddler">Jack N. Saddler</name></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="eISSN">1097-0290</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Biotechnology (methods)</term><term>Carboxylic Acids (analysis)</term><term>Cellulases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (isolation & purification)</term><term>Lignin (metabolism)</term><term>Magnetic Resonance Spectroscopy (MeSH)</term><term>Pinus (chemistry)</term><term>Pinus (metabolism)</term><term>Populus (chemistry)</term><term>Populus (metabolism)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Zea mays (chemistry)</term><term>Zea mays (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides carboxyliques (analyse)</term><term>Biomasse (MeSH)</term><term>Biotechnologie (méthodes)</term><term>Cellulases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (isolement et purification)</term><term>Lignine (métabolisme)</term><term>Pinus (composition chimique)</term><term>Pinus (métabolisme)</term><term>Populus (composition chimique)</term><term>Populus (métabolisme)</term><term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term><term>Spectroscopie par résonance magnétique (MeSH)</term><term>Zea mays (composition chimique)</term><term>Zea mays (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carboxylic Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulases</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Acides carboxyliques</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulases</term><term>Lignine</term><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Hydrolysis</term><term>Magnetic Resonance Spectroscopy</term><term>Spectroscopy, Fourier Transform Infrared</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Hydrolyse</term><term>Spectroscopie infrarouge à transformée de Fourier</term><term>Spectroscopie par résonance magnétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To assess the effects that the physical and chemical properties of lignin might have on the enzymatic hydrolysis of pretreated lignocellulosic substrates, protease treated lignin (PTL) and cellulolytic enzyme lignin (CEL) fractions, isolated from steam and organosolv pretreated corn stover, poplar, and lodgepole pine, were prepared and characterized. The adsorption of cellulases to the isolated lignin preparations corresponded to a Langmuir adsorption isotherm. It was apparent that, rather than the physical properties of the isolated lignin, the carboxylic acid functionality of the isolated lignin, as determined by FTIR and NMR spectroscopy, had much more of an influence when lignin was added to typical hydrolysis of pure cellulose (Avicel). An increase in the carboxylic content of the lignin preparation resulted in an increased hydrolysis yield. These results suggested that the carboxylic acids within the lignin partially alleviate non-productive binding of cellulases to lignin. To try to confirm this possible mechanism, dehydrogenative polymers (DHP) of monolignols were synthesized from coniferyl alcohol (CA) and ferulic acid (FA), and these model compounds were added to a typical enzymatic hydrolysis of Avicel. The DHP from FA, which was enriched in carboxylic acid groups compared with the DHP from CA, adsorbed a lower mount of cellulases and did not decrease hydrolysis yields when compared to the DHP from CA, which decreased the hydrolysis of Avicel by 8.4%. Thus, increasing the carboxylic acid content of the lignin seemed to significantly decrease the non-productive binding of cellulases and consequently increased the enzymatic hydrolysis of the cellulose.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21246506</PMID><DateCompleted><Year>2011</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2011</Year><Month>01</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-0290</ISSN><JournalIssue CitedMedium="Internet"><Volume>108</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin.</ArticleTitle><Pagination><MedlinePgn>538-48</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/bit.22981</ELocationID><Abstract><AbstractText>To assess the effects that the physical and chemical properties of lignin might have on the enzymatic hydrolysis of pretreated lignocellulosic substrates, protease treated lignin (PTL) and cellulolytic enzyme lignin (CEL) fractions, isolated from steam and organosolv pretreated corn stover, poplar, and lodgepole pine, were prepared and characterized. The adsorption of cellulases to the isolated lignin preparations corresponded to a Langmuir adsorption isotherm. It was apparent that, rather than the physical properties of the isolated lignin, the carboxylic acid functionality of the isolated lignin, as determined by FTIR and NMR spectroscopy, had much more of an influence when lignin was added to typical hydrolysis of pure cellulose (Avicel). An increase in the carboxylic content of the lignin preparation resulted in an increased hydrolysis yield. These results suggested that the carboxylic acids within the lignin partially alleviate non-productive binding of cellulases to lignin. To try to confirm this possible mechanism, dehydrogenative polymers (DHP) of monolignols were synthesized from coniferyl alcohol (CA) and ferulic acid (FA), and these model compounds were added to a typical enzymatic hydrolysis of Avicel. The DHP from FA, which was enriched in carboxylic acid groups compared with the DHP from CA, adsorbed a lower mount of cellulases and did not decrease hydrolysis yields when compared to the DHP from CA, which decreased the hydrolysis of Avicel by 8.4%. Thus, increasing the carboxylic acid content of the lignin seemed to significantly decrease the non-productive binding of cellulases and consequently increased the enzymatic hydrolysis of the cellulose.</AbstractText><CopyrightInformation>Copyright © 2010 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakagame</LastName><ForeName>Seiji</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chandra</LastName><ForeName>Richard P</ForeName><Initials>RP</Initials></Author><Author ValidYN="Y"><LastName>Kadla</LastName><ForeName>John F</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Saddler</LastName><ForeName>Jack N</ForeName><Initials>JN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>11</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002264">Carboxylic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D044602">Cellulases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002264" MajorTopicYN="N">Carboxylic Acids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044602" MajorTopicYN="N">Cellulases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009682" MajorTopicYN="N">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>08</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>10</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>10</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21246506</ArticleId><ArticleId IdType="doi">10.1002/bit.22981</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Chandra, Richard P" sort="Chandra, Richard P" uniqKey="Chandra R" first="Richard P" last="Chandra">Richard P. Chandra</name><name sortKey="Kadla, John F" sort="Kadla, John F" uniqKey="Kadla J" first="John F" last="Kadla">John F. Kadla</name><name sortKey="Saddler, Jack N" sort="Saddler, Jack N" uniqKey="Saddler J" first="Jack N" last="Saddler">Jack N. Saddler</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Nakagame, Seiji" sort="Nakagame, Seiji" uniqKey="Nakagame S" first="Seiji" last="Nakagame">Seiji Nakagame</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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